Bsp1720 I

Enzyme nameBsp1720 I
PrototypeEspI
Known analogsBlpI, Bpu1102I, CelII, EspI
SKUSE-E185
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GC▼TNAGC …3'
3'… CGANT▲CG …5'
SourceBacillus species 1720
Optimal bufferSE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
5010050507575
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme about 80% of DNA fragment can be ligated. Of these about 95% can be recut
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer G
References
  1. S.Kh. Degtyarev, A.A. Kolyhalov, N.I. Rechkunova, I.I. Tepavicharova, L.I. Mechandjiska, E.I. Builieva Bsp1720 I, an isoschizomer of Esp I from Bacillus species 1720 recognizing 5′-GC^TNAGC-3′. // Nucleic Acids Research, Vol. 19, No. 9, 2504 (1991).
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