Bst2U I

Enzyme name Bst2U I
Prototype BstNI
Known analogs BptI, BseBI, BstNI, BstOI, MvaI, Ajn I, EcoRII, Psp6 I, PspGI
SKU SE-E051
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CC▼WGG …3'
3'… GGW▲CC …5'
Source Bacillus stearothermophilus 2U
Optimal buffer SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature 60 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
7510050501050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivity Not blocked by overlapping Dcm methylation(C m CWGG) CCWGG.
Reagents supplied with enzyme 10 X SE-buffer G, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  6. Unpublished data Myakisheva, T.V., Degtyarev, S.K Unpublished observations (1995).
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