BstFN I

Enzyme nameBstFN I
PrototypeFnuDII
Known analogsAccII, Bsh1236I, BstUI, FnuDII, MvnI
SKUSE-E283
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CG▼CG …3'
3'… GC▲GC …5'
SourceBacillus stearothermophilus FN
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature60 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
7550252510075
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions20 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 10 mM MgCl 2 200 μg/ml BSA, 7 mM 2-mercaptoethanol and 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivityBlocked by CG methylation
Reagents supplied with enzyme10 X SE-buffer Y
References
  4. Unpublished data Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K Unpublished observations (1999).
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