BstFN I

Enzyme name BstFN I
Prototype FnuDII
Known analogs AccII, Bsh1236I, BstUI, FnuDII, MvnI
SKU SE-E283
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CG▼CG …3'
3'… GC▲GC …5'
Source Bacillus stearothermophilus FN
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 60 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
7550252510075
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 20 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 10 mM MgCl 2 200 μg/ml BSA, 7 mM 2-mercaptoethanol and 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivity Blocked by CG methylation
Reagents supplied with enzyme 10 X SE-buffer Y
Notes
References
  4. Unpublished data Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K Unpublished observations (1999).
// Код для некролога