Dri I

Enzyme nameDri I
PrototypeEam1105I
Known analogsAhdI, AspEI, Eam1105I, EclHKI
SKUSE-E193
Turbo versionAvailable
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GACNNN▼NNGTC …3'
3'… CTGNN▲NNNCAG …5'
SourceDeinococcus radiodurans EA
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature65 °C
Buffer activity
BGOWYROSE
7575101010040
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
References
  1. Unpublished data Abdurashitov, M.A., Nayakshina, T.N., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K Unpublished observations (2003)
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