Hae III

Enzyme name Hae III
Prototype HaeIII
Known analogs BshFI, BspANI, BsuR I, PhoI
SKU SE-E067
Turbo version Available
High-concentration version Available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GG▼CC …3'
3'… CC▲GG …5'
Source An E.coli strain, that carries the cloned Hae III gene from Haemophilus aegyptius
Optimal buffer SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75100255050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by methylation: 5`-GG(5mC)C-3`/ 3`-C(5mC)GG-5`.
Reagents supplied with enzyme 10 X SE-buffer G
Notes
References
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