Hae III

1,100.00 4,400.00 

  • CNY: 89.45 ¥ - 357.81 ¥

Restriction endonuclease Hae III

  • Recognition site: GG↑CC / CC↓GG
  • Источник: Из штамма E.coli несущего клонированный ген HaeIII из Haemophilus aegyptius
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E067 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E067TS2000 uTurbo10 000 u/ml1,100.00 
  • CNY: 89.45 ¥
E068TL10000 uTurbo10 000 u/ml4,400.00 
  • CNY: 357.81 ¥
E067S2000 uRegular10 000 u/ml1,100.00 
  • CNY: 89.45 ¥
E068L10000 uRegular10 000 u/ml4,400.00 
  • CNY: 357.81 ¥
E068XL10000 uConcentrated50 000 u/ml4,400.00 
  • CNY: 357.81 ¥

Description

Recognition site and hydrolysis position:

GGCC GG↑CC
CCGG CC↓GG

Source: An E.coli strain, that carries the cloned Hae III gene from Haemophilus aegyptius
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
75 100 25 50 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by methylation: 5`-GG(5mC)C-3`/ 3`-C(5mC)GG-5`.
Notes:
References:
Middleton, J.H., Edgell, M.H., Hutchison, C.A. J. Virol. 10:42-50 (1972).
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006
M.A. Abdurashitov, V.N. Tomilov, V.A. Chernukhin, D.A. Gonchar, S.Kh. Degtyarev Mammalian chromosomal DNA digestion with restriction endonucleases in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 29-38, 2006