Mab I

Enzyme name Mab I
Prototype SexAI
Known analogs SexAI
SKU SE-E121
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… A▼CCWGGT …3'
3'… TGGWCC▲A …5'
Source Microbacterium arborescens SE
Optimal buffer SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25507510050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity Not blocked by overlapping Dcm methylation (C m CWGG) A CCWGG T.
Reagents supplied with enzyme 10 X SE-buffer W, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Unpublished data Nadeev, A.N., Tsaplin, M.M., Abdurashitov, M.A., Dedkov, V.S., Kashirina, J.G., Popichenko, D.V., Degtyarev, S.K Unpublished observations (2001).
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