Mab I

6,000.00 24,000.00 

  • CNY: 487.92 ¥ - 1,951.68 ¥

Restriction endonuclease Mab I

  • Recognition site: A↑CCWGGT / TGGWCC↓A
  • Источник: Microbacterium arborescens SE
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E121 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E121S200 uRegular1 000-5 000 u/ml6,000.00 
  • CNY: 487.92 ¥
E122L1000 uRegular1 000-5 000 u/ml24,000.00 
  • CNY: 1,951.68 ¥

Description

Recognition site and hydrolysis position:

ACCWGGT A↑CCWGGT
TGGWCCA TGGWCC↓A

Source: Microbacterium arborescens SE
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
25 50 75 100 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer W, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by overlapping Dcm methylation (CmCWGG) ACCWGGT.
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Nadeev, A.N., Tsaplin, M.M., Abdurashitov, M.A., Dedkov, V.S., Kashirina, J.G., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).