Mal I

Enzyme nameMal I
PrototypeDpnI
Known analogsDpnI
SKUSE-E489
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIMiType IIM enzymes require a modified, usually methylated, recognition site for cleavage., Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… G(mA)▼TC …3'
3'… CT▲(mA)G …5'
SourceMarinococus albus I
Optimal bufferSE-buffer MalI (20 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature65 °C
Buffer activity
BGOWYROSE
252550755050
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA (dam-methylated) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onpBR322 DNA (dam-methylated)
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme more than 20% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivityThe enzyme cleaves only methylated DNA.
Reagents supplied with enzyme10 X SE-buffer MalI
References

No publications found.

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