MspR9 I

Enzyme name MspR9 I
Prototype ScrFI
Known analogs Bme1390I, ScrFI, BssKI, BstSC I, StyD4I
SKU SE-E175
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CC▼NGG …3'
3'… GGN▲CC …5'
Source Micrococcus species R9
Optimal buffer SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
50501005050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dcm-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoetyanol; 100 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dcm methylation(C m CWGG) CCAGG and CCTGG.
Reagents supplied with enzyme 10 X SE-buffer O
Notes
References

No publications found.
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