MspR9 I

Enzyme nameMspR9 I
PrototypeScrFI
Known analogsBme1390I, ScrFI, BssKI, BstSC I, StyD4I
SKUSE-E175
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CC▼NGG …3'
3'… GGN▲CC …5'
SourceMicrococcus species R9
Optimal bufferSE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature37 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
50501005050100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed onLambda DNA (dcm-)
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoetyanol; 100 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivityBlocked by overlapping Dcm methylation(C m CWGG) CCAGG and CCTGG.
Reagents supplied with enzyme10 X SE-buffer O
References

No publications found.

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