PspOM I

Enzyme name PspOM I
Prototype (ApaI)
Known analogs Bsp120I, Apa I
SKU SE-E215
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… G▼GGCCC …3'
3'… CCCGG▲G …5'
Source An E.coli strain that carries the cloned PspOM I gene from Pseudomonas species OM2164
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75100010025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (BamHI-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (BamHI-digest)
Storage conditions 20 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer Y
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13.
References
  1. Unpublished data Belichenko, O.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh Unpublished observations (1994).
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