Tth111 I

Enzyme nameTth111 I
PrototypeTth111I
Known analogsAspI, PflFI, PsyI, TelI
SKUSE-E097
Turbo versionNot available
High-concentration versionNot available
Restriction typeType IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GACN▼NNGTC …3'
3'… CTGNN▲NCAG …5'
SourceAn E.coli strain, that carries the cloned gene Tth111I from Thermus Thermophilus 111
Optimal bufferSE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature65 °C
Inactivation temperature80 °C
Buffer activity
BGOWYROSE
75501010100100
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed onLambda DNA (HindIII-digest)
Storage conditions10 mM Tris-HCl (pH 7.5); 500 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
LigationAfter 2-fold overdigestion with enzyme 10% of the DNA fragments can be ligated.
Nonspecific hydrolysisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivitynot tested
Reagents supplied with enzyme10 X SE-buffer Y
NotesHigh enzyme concentrationmay result in star activity.
References
  1. External publication Shinomiya, T., Sato, S. Nucleic Acids Res. 8: 43-56 (1980).
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