Tth111 I

Enzyme name Tth111 I
Prototype Tth111I
Known analogs AspI, PflFI, PsyI, TelI
SKU SE-E097
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… GACN▼NNGTC …3'
3'… CTGNN▲NCAG …5'
Source An E.coli strain, that carries the cloned gene Tth111I from Thermus Thermophilus 111
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75501010100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 500 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligation After 2-fold overdigestion with enzyme 10% of the DNA fragments can be ligated.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer Y
Notes High enzyme concentrationmay result in star activity.
References
  1. External publication Shinomiya, T., Sato, S. Nucleic Acids Res. 8: 43-56 (1980).
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