Xma I

Enzyme name Xma I
Prototype XmaI
Known analogs Cfr9I, XmaCI, Sma I
SKU SE-E233
Turbo version Not available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… C▼CCGGG …3'
3'… GGGCC▲C …5'
Source An E.coli strain, that carries the cloned gene XmaI from Xanthomonas malvacearum
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75500010050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus -2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Adenovirus -2 DNA
Storage conditions 20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer Y
Notes
References

No publications found.
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