AsiG I

2,090.00 ₽ – 8,360.00 ₽

  • CNY: 169.96 ¥ - 679.84 ¥

Restriction endonuclease AsiG I

  • Recognition site: A↑CCGGT / TGGCC↓A
  • Источник: Arthrobacter species G
  • Оптимальный буфер: SE-буфер O
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E235 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E235S100 uRegular3 000-5 000 u/ml2,090.00 ₽
  • CNY: 169.96 ¥
E236L500 uRegular3 000-5 000 u/ml8,360.00 ₽
  • CNY: 679.84 ¥
E235TS100 uTurbo3 000-5 000 u/ml2,090.00 ₽
  • CNY: 169.96 ¥
E236TL500 uTurbo3 000-5 000 u/ml8,360.00 ₽
  • CNY: 679.84 ¥

Description

Recognition site and hydrolysis position:

ACCGGT A↑CCGGT
TGGCCA TGGCC↓A

Source: Arthrobacter species G
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

B G O W Y ROSE
10 25 100 75 10 75

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: Turbo AsiG I can be used for short time (10 min) DNA digestion as well as for standard reaction. The reaction can be performed using optimal or universal (ROSE) Buffer.
References:
Chmuzh, E.V., Mikhnenkova, N.A., Nayakshina, T.N., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations.(2005)

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