AspLE I

1,100.00 ₽ – 4,400.00 ₽

  • CNY: 89.45 ¥ - 357.81 ¥

Restriction endonuclease AspLE I

  • Recognition site: GCG↑C / C↓GCG
  • Источник: Arthrobacter species LE3860
  • Оптимальный буфер: SE-буфер O
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: N
SKU: SE-E221 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E221TS500 uTurbo10 000 u/ml1,100.00 ₽
  • CNY: 89.45 ¥
E222TL2500 uTurbo10 000 u/ml4,400.00 ₽
  • CNY: 357.81 ¥
E221S500 uRegular10 000 u/ml1,100.00 ₽
  • CNY: 89.45 ¥
E222L2500 uRegular10 000 u/ml4,400.00 ₽
  • CNY: 357.81 ¥

Description

Recognition site and hydrolysis position:

GCGC GCG↑C
CGCG C↓GCG

Source: Arthrobacter species LE3860
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

B G O W Y ROSE
10 75 100 50 25 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by
5′-G(5mC)GC-3’/3-CG(5mC)G-5′ methylation.
Not blocked by
5′-GCG(5mC)-3’/3`-(5mC)GCG-5` or
5′-GCG(5mC)-3’/3′-CGCG-5’methylation.
Cut hemimethylated site: 5′-G(5mC)GC-3’/3′-CGCG-5′
Notes:
References:
Vysotskaya, E.M., Gonchar, D.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1998).

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