Description
Recognition Site and Hydrolysis Position:
5'… GG▼GWCCC …3'
3'… CCCWG▲GG …5'
Source: Brevundimonas mongoliensis 53
Definition of Activity Unit:
Substrate for Activity Determination:
T7 DNA
Optimal Buffer: SE buffer W
Enzyme Activity in Various Buffers as a Percentage of Maximum:
| B | G | O | W | Y | ROSE |
| 25-50 | 50-75 | 25-50 | 100 | 50-75 | 75 |
Storage Conditions: ; Store at -20°C
Ligase Compatibility:
Non-Specific Hydrolysis:
Methylation Sensitivity:
Supplied with the Enzyme: 10 x SE Buffer W
