Description
Recognition Site and Hydrolysis Position:
Source: Brevundimonas mongoliensis 53
Definition of Activity Unit: One unit is defined as the amount of enzyme required to digest 1 µg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Substrate for Activity Determination: T7 DNA
Optimal Buffer: SE buffer W
Enzyme Activity in Various Buffers as a Percentage of Maximum:
| B | G | O | W | Y | ROSE |
| 25-50 | 50-75 | 25-50 | 100 | 50-75 | 75 |
Storage Conditions: 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol; Store at -20°C
Ligase Compatibility: After 3-fold overdigestion with Bmo I, >90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-Specific Hydrolysis: A 50 µl reaction containing 1 µg of DNA and 4 units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour
Oligonucleotide Assay: No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 2 units of restriction endonuclease for 3 hours.
Methylation Sensitivity: Blocked by overlapping CG-methylation GGGWCC(5mC)G
Supplied with the Enzyme: 10x SE Buffer W
Enzyme Properties: When using a buffer other than the optimal (supplied) SE-Buffer, it may be necessary to add more enzyme to achieve complete digestion.
