Bso31 I

Bso31 I

Price range: 1 500 ₽ through 6 000 ₽

Restriction endonuclease Bso31 I

  • Recognition site:
    5'… GGTCTCN▼ …3'
    3'… CCAGAG(N)5▲ …5'
  • Source: Bacillus stearothermophilus 31
  • Optimal buffer: SE-buffer O
  • Optimal temperature: 55 °C
  • Inactivated during 20 minutes at: 80 °C
SKU: SE-E285 Category:

SKUРackagePackageVariantConcentrationPrice 
E285TS200 uTurbo5 000-10 000 u/ml1 500 ₽
E286TL1000 uTurbo5 000-10 000 u/ml6 000 ₽
E285S200 uRegular5 000-10 000 u/ml1 500 ₽
E286L1000 uRegular5 000-10 000 u/ml6 000 ₽

Description

Recognition site and hydrolysis position:

GGTCTCN GGTCTCN↑
CCAGAG(N)5 CCAGAG(N)5↓

Source: Bacillus stearothermophilus 31
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
T7 DNA
Optimal SE-buffer: SE-buffer O + BSA
Enzyme activity (%):

B G O W Y ROSE
25 75 100 75 25 40

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated. Of these 80% can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 10 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer O, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dcm-methylation (CmCWGG): GAGACCWGG
Not blocked by methylation GGTCT(5mC)
Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Shinkarenko, N.M., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1999).

Additional Information

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