BstAC I

2,300.00 9,200.00 

  • CNY: 179.38 ¥ - 717.51 ¥

Restriction endonuclease BstAC I

  • Recognition site: GR↑CGYC / CYGC↓RG
  • Источник: Bacillus stearothermophilus AC
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E093 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E093TS500 uTurbo10 000 u/ml2,300.00 
  • CNY: 179.38 ¥
E094TL2500 uTurbo10 000 u/ml9,200.00 
  • CNY: 717.51 ¥
E093S500 uRegular10 000 u/ml2,300.00 
  • CNY: 179.38 ¥
E094L2500 uRegular10 000 u/ml9,200.00 
  • CNY: 717.51 ¥

Description

Recognition site and hydrolysis position:

GRCGYC GR↑CGYC
CYGCRG CYGC↓RG

Source: Bacillus stearothermophilus AC
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
75 75 50 100 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Belichenko, O.A., Schevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1996).