BstDS I

BstDS I

Price range: 7 140,00 ₽ through 28 560,00 ₽ в т.ч. НДС 5%

Restriction endonuclease BstDS I

  • Recognition site:
    5'… C▼CRYGG …3'
    3'… GGYRC▲C …5'
  • Source: Bacillus stearothermophilus DS
  • Optimal buffer: SE-buffer Y
  • Optimal temperature: 65 °C
  • Inactivated during 20 minutes at: 80 °C
SKU: SE-E083 Category:

SKUРackagePackageVariantConcentrationPrice 
E083S1000 uRegular10 000 u/ml7 140,00 ₽ в т.ч. НДС 5%
E084L5000 uRegular10 000 u/ml28 560,00 ₽ в т.ч. НДС 5%

Description

Recognition site and hydrolysis position:

CCRYGG C↑CRYGG
GGYRCC GGYRC↓C

Source: Bacillus stearothermophilus DS
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
0 75 50 25 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).

Additional Information

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