4,350.00 17,400.00 

  • CNY: 339.26 ¥ - 1,357.03 ¥

Restriction endonuclease BstSC I

  • Recognition site: ↑CCNGG / GGNCC↓
  • Источник: Bacillus stearothermophilus SC
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 80
SKU: SE-E307 Category:

Available Options

E307S100 uRegular2 000 - 5 000 u/ml4,350.00 
  • CNY: 339.26 ¥
E308L500 uRegular2 000 - 5 000 u/ml17,400.00 
  • CNY: 1,357.03 ¥


Recognition site and hydrolysis position:


Source: Bacillus stearothermophilus SC
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dcm-)
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

50 50 50 50 100 60

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by Dcm-methylation (CmCWGG) CCWGG.
Notes: At 37°C activity is 10% from maximum.
Dedkov, V.S., Muradov, S.V., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from “Medical genetics” V.6, No 8, pp 29-36, 2007