BsuR I

1,125.00 ₽ – 4,500.00 ₽

  • CNY: 91.49 ¥ - 365.94 ¥

Restriction endonuclease BsuR I

  • Recognition site: GG↑CC / CC↓GG
  • Источник: Bacillus subtilis R
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E053 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E053S1000 uRegular20 000 u/ml1,125.00 ₽
  • CNY: 91.49 ¥
E054L5000 uRegular20 000 u/ml4,500.00 ₽
  • CNY: 365.94 ¥

Description

Recognition site and hydrolysis position:

GGCC GG↑CC
CCGG CC↓GG

Source: Bacillus subtilis R
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
75 100 25 50 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Bron, S., Murray, K., Trautner, T.A. Mol. Gen. Genet. 143: 13-23 (1975).

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