Dri I

1,150.00 ₽ – 4,600.00 ₽

  • CNY: 93.52 ¥ - 374.07 ¥

Restriction endonuclease Dri I

  • Recognition site: GACNNN↑NNGTC / CTGNN↓NNNCAG
  • Источник: Deinococcus radiodurans EA
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E193 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E193TS500 uTurbo10 000 u/ml1,150.00 ₽
  • CNY: 93.52 ¥
E194TL2500 uTurbo10 000 u/ml4,600.00 ₽
  • CNY: 374.07 ¥
E193S500 uRegular10 000 u/ml1,150.00 ₽
  • CNY: 93.52 ¥
E194L2500 uRegular10 000 u/ml4,600.00 ₽
  • CNY: 374.07 ¥

Description

Recognition site and hydrolysis position:

GACNNNNNGTC GACNNN↑NNGTC
CTGNNNNNCAG CTGNN↓NNNCAG

Source: Deinococcus radiodurans EA
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 75 10 10 100 40

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated. Ligation is better in presence of 10% PEG.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Abdurashitov, M.A., Nayakshina, T.N., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2003)

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