Description
| Enzyme name | EmiS6 I | ||||||||||||
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| Prototype | Ksp632I | ||||||||||||
| SKU | SE-E609 | ||||||||||||
| Turbo version | Not available | ||||||||||||
| High-concentration version | Not available | ||||||||||||
| Recognition site | 5'… CTCTTC(N)1▼ …3' 3'… GAGAAG(N)4▲ …5' |
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| Source | Exiguobacterium mexicanum 6 Definition of Activity Unit One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Substrate for Activity Determination: λ DNA | ||||||||||||
| Optimal buffer | SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) | ||||||||||||
| Optimal temperature | 37 °C | ||||||||||||
| Inactivation temperature | 80 °C | ||||||||||||
| Buffer activity |
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| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | ||||||||||||
| Assayed on | Lambda DNA | ||||||||||||
| Storage conditions | 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol Store at -20°C | ||||||||||||
| Ligation | After digestion with a 5-fold excess of enzyme, 95% of DNA fragments can be ligated by DNA ligase, and 80% of these can be recut. | ||||||||||||
| Nonspecific hydrolysis | A 50 µl reaction containing 1 µg of DNA and 5 units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour. Oligonucleotide Assay No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 5 units of restriction endonuclease for 3 hours. | ||||||||||||
| Methylation sensitivity | |||||||||||||
| Reagents supplied with enzyme | 10x SE Buffer Y | ||||||||||||
| Notes | |||||||||||||
| References |
