Fat I

4,070.00 16,280.00 

  • CNY: 314.65 ¥ - 1,258.61 ¥

Restriction endonuclease Fat I

  • Recognition site: ↑CATG / GTAC↓
  • Источник: Из штамма E.coli несущего клонированный ген Fat I из Flavobacterium aquatile NL3
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 65
SKU: SE-E155 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E155S100 uRegular2 000 - 5 000 u/ml4,070.00 
  • CNY: 314.65 ¥
E156L500 uRegular2 000 - 5 000 u/ml16,280.00 
  • CNY: 1,258.61 ¥

Description

Recognition site and hydrolysis position:

CATG ↑CATG
GTAC GTAC↓

Source: An E.coli strain that carries the cloned Fat I gene from Flavobacterium aquatile NL3
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
pUC19 DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
10 100 25 10 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 3 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by mCATG methylation
Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.
FatI cleaves linear plasmid DNA at a rate 1.5-2 times higher than supercoiled plasmid DNA.
References:
Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Biotekhnologia #5, 3-7 (2002)
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006