Fau I

4,500.00 ₽ – 18,000.00 ₽

  • CNY: 349.97 ¥ - 1,399.86 ¥

Restriction endonuclease Fau I

  • Recognition site: CCCGC(N)4↑ / GGGCG(N)6↓
  • Источник: Из штамма E.coli несущего клонированный ген Fau I из Flavobacterium aquatili
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 65
SKU: SE-E209 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E209S100 uRegular2 000 u/ml4,500.00 ₽
  • CNY: 349.97 ¥
E210L500 uRegular2 000 u/ml18,000.00 ₽
  • CNY: 1,399.86 ¥

Description

Recognition site and hydrolysis position:

CCCGC(N)4 CCCGC(N)4↑
GGGCG(N)6 GGGCG(N)6↓

Source: An E.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 25 0 0 50 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycero; Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and of these 95% can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 4 u.a. of enzyme for 16 hours at 55°C Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CG methylation.
Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
References:
Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova,N.I., Dedkov, V.S. Bioorg. Khim. 15: 130-132 (1989).

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