HpySE526 I

2,300.00 ₽ – 9,200.00 ₽

  • CNY: 178.87 ¥ - 715.48 ¥

Restriction endonuclease HpySE526 I

  • Recognition site: A↑CGT / TGC↓A
  • Источник: Из штамма E.coli несущего клонированный ген HpySE 526I из Helicobacter pylori SE526
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E583 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E583S200 uRegular5 000 u/ml2,300.00 ₽
  • CNY: 178.87 ¥
E584L1000 uRegular5 000 u/ml9,200.00 ₽
  • CNY: 715.48 ¥

Description

Recognition site and hydrolysis position:

ACGT A↑CGT
TGCA TGC↓A

Source: An E.coli strain, that carries the cloned HpySE 526I gene from Helicobacter pylori SE526.
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
pUC19 DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 75 10 25 100 50

Storage conditions: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by CG methylation
Notes:
References:

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