Lmn I

6,600.00 ₽ – 26,400.00 ₽

  • CNY: 536.71 ¥ - 2,146.85 ¥

Restriction endonuclease Lmn I

  • Recognition site: GCTCCN↑ / CGAG↓GN
  • Источник: Lysinibacillus manganicus An22
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E593 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E593S50 uRegular500-1 000 u/ml6,600.00 ₽
  • CNY: 536.71 ¥
E594L250 uRegular500-1 000 u/ml26,400.00 ₽
  • CNY: 2,146.85 ¥

Description

Recognition site and hydrolysis position:

GCTCCN GCTCCN↑
CGAGGN CGAG↓GN

Source: Lysinibacillus manganicus An22
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 75 50 50 75 80

Storage conditions: 20 mM Tris-HCl (pH 7.5); 250 mM NaCl; 100 μg/ml BSA; 7 mM 2-mercaptoethanol, 50% glycerol. Store at -20ºC.
Ligations:
After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37ºC. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity:
Notes:
References:

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