Mly113 I

Mly113 I

Price range: 5 000 ₽ through 20 000 ₽

Restriction endonuclease Mly113 I

  • Recognition site:
    5'… GG▼CGCC …3'
    3'… CCGC▲GG …5'
  • Source: Micrococcus lylae 113
  • Optimal buffer: SE-buffer B
  • Optimal temperature: 37 °C
  • Inactivated during 20 minutes at: 65 °C
SKU: SE-E189 Category:

SKUРackagePackageVariantConcentrationPrice 
E189S200 uRegular2 000 u/ml5 000 ₽
E190L1000 uRegular2 000 u/ml20 000 ₽

Description

Recognition site and hydrolysis position:

GGCGCC GG↑CGCC
CCGCGG CCGC↓GG

Source: Micrococcus lylae 113
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
T7 DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 25 10 10 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
References:
Dedkov, V.S., Zernov, Y.P., Rechkunova, N.I., Degtyarev, S.Kh. Mol.Genet.Mikrobiol.Virusol. 12: 17-18 (1990).

Additional Information

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