Mly113 I

4,050.00 ₽ – 16,200.00 ₽

  • CNY: 329.35 ¥ - 1,317.38 ¥

Restriction endonuclease Mly113 I

  • Recognition site: GG↑CGCC / CCGC↓GG
  • Источник: Micrococcus lylae 113
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E189 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E189S200 uRegular2 000 u/ml4,050.00 ₽
  • CNY: 329.35 ¥
E190L1000 uRegular2 000 u/ml16,200.00 ₽
  • CNY: 1,317.38 ¥

Description

Recognition site and hydrolysis position:

GGCGCC GG↑CGCC
CCGCGG CCGC↓GG

Source: Micrococcus lylae 113
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
T7 DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 25 10 10 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
References:
Dedkov, V.S., Zernov, Y.P., Rechkunova, N.I., Degtyarev, S.Kh. Mol.Genet.Mikrobiol.Virusol. 12: 17-18 (1990).

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