MroN I

3,750.00 ₽ – 15,000.00 ₽

  • CNY: 304.95 ¥ - 1,219.80 ¥

Restriction endonuclease MroN I

  • Recognition site: G↑CCGGC / CGGCC↓G
  • Источник: Micrococcus roseus N0
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E087 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E087S500 uRegular5 000 u/ml3,750.00 ₽
  • CNY: 304.95 ¥
E088L2500 uRegular5 000 u/ml15,000.00 ₽
  • CNY: 1,219.80 ¥

Description

Recognition site and hydrolysis position:

GCCGGC G↑CCGGC
CGGCCG CGGCC↓G

Source: Micrococcus roseus N0
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Adenovirus-2 DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 50 10 0 10 5

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the Ad-2 DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Dedkov, V.S., Belichenko, O.A., Prikhod’ko, E.A., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1994).

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