Psp124B I

2,200.00 ₽ – 8,800.00 ₽

  • CNY: 178.90 ¥ - 715.62 ¥

Restriction endonuclease Psp124B I

  • Recognition site: GAGCT↑C / C↓TCGAG
  • Источник: Pseudomonas species 124B
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E107 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E107S1000 uRegular20 000 u/ml2,200.00 ₽
  • CNY: 178.90 ¥
E108L5000 uRegular20 000 u/ml8,800.00 ₽
  • CNY: 715.62 ¥
E107TS1000 uTurbo20 000 u/ml2,200.00 ₽
  • CNY: 178.90 ¥
E108TL5000 uTurbo20 000 u/ml8,800.00 ₽
  • CNY: 715.62 ¥

Description

Recognition site and hydrolysis position:

GAGCTC GAGCT↑C
CTCGAG C↓TCGAG

Source: Pseudomonas species 124B
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (HindIII-digest)
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
75 100 10 0 75 30

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Degtyarev, S.Kh., Dedkov, V.S., Rechkunova, N.I., Morgan, R. Unpublished observations (1994).

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