Psp6 I

1,950.00 ₽ – 7,800.00 ₽

  • CNY: 150.62 ¥ - 602.47 ¥

Restriction endonuclease Psp6 I

  • Recognition site: ↑CCWGG / GGWCC↓
  • Источник: Pseudomonas species 6
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 80
SKU: SE-E453 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E453S100 uRegular1 000-3 000 u/ml1,950.00 ₽
  • CNY: 150.62 ¥
E454L500 uRegular1 000-3 000 u/ml7,800.00 ₽
  • CNY: 602.47 ¥

Description

Recognition site and hydrolysis position:

CCWGG ↑CCWGG
GGWCC GGWCC↓

Source: Pseudomonas species 6
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (dcm-)
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 50 10 25 75 10

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol.
Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 4 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by overlapping Dcm methylation(CmCWGG): CCWGG.
Notes:
References:
Dedkov, V.S., Tomilova, J.E., Kileva, E.V., Degtyarev, S.K. Unpublished observations. (2002)
V.S. Dedkov, N.A. Mikhnenkova, L.P. Vlasenko, J.E. Tomilova, J.G.Kashirina, D.A. Gonchar, S.Kh. Degtyarev Restriction Endonuclease AjnI from Acinetobacter johnsonii R2, an Isoschizomer of EcoRII, recognizes 5′- CCWGG-3′ and cleaves dcm-methylated sites // Translated from BIOTEHNOLOGIA (Russia) No. 3, pp. 19-24, 2004

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