PspOM I

2,530.00 ₽ – 10,120.00 ₽

  • CNY: 198.98 ¥ - 795.94 ¥

Restriction endonuclease PspOM I

  • Recognition site: G↑GGCCC / CCCGG↓G
  • Источник: Из штамма E.coli несущего клонированный ген PspOM I из Pseudomonas species OM2164
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E215 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E215S1500 uRegular10 000 u/ml2,530.00 ₽
  • CNY: 198.98 ¥
E216L7500 uRegular10 000 u/ml10,120.00 ₽
  • CNY: 795.94 ¥

Description

Recognition site and hydrolysis position:

GGGCCC G↑GGCCC
CCCGGG CCCGG↓G

Source: An E.coli strain that carries the cloned PspOM I gene from Pseudomonas species OM2164
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (BamHI-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (BamHI-digest)
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 10 0 0 100 25

Storage conditions: 20 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13.
References:
Belichenko, O.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1994).

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