Psr I

5,500.00 ₽ – 22,000.00 ₽

  • CNY: 447.26 ¥ - 1,789.04 ¥

Restriction endonuclease Psr I

  • Recognition site: ↑(N)7GAACNNNNNNTAC(N)12↑ / ↓21(N)CTTGNNNNNNATG7(N)↓
  • Источник: Pseudomonas stutzeri N2
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 30
  • Температура инактивации, 20 мин при: 65
SKU: SE-E131 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E131S100 uRegular1 000-3 000 u/ml5,500.00 ₽
  • CNY: 447.26 ¥
E132L500 uRegular1 000-3 000 u/ml22,000.00 ₽
  • CNY: 1,789.04 ¥

Description

Recognition site and hydrolysis position:

(N)7GAACNNNNNNTAC(N)12 ↑(N)7GAACNNNNNNTAC(N)12↑
(N)12CTTGNNNNNNATG(N)7 ↓(N)12CTTGNNNNNNATG(N)7↓

Source: Pseudomonas stutzeri N2
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 30°C in a total reaction volume of 50 μl.
Assayed on:
T7 DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
10 10 0 0 100 30

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme more than 70% of DNA fragments can be ligated. Of these 80% can be recut. In the presence of 10% PEG ligation is better.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 30°C. Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity.
Incubation at 37°C results in 20% activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Gonchar, D.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).

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