PstN I

5,500.00 ₽ – 22,000.00 ₽

  • CNY: 429.49 ¥ - 1,717.98 ¥

Restriction endonuclease PstN I

  • Recognition site: CAGNNN↑CTG / GTC↓NNNGAC
  • Источник: Pseudomonas stutzeri 217
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E571 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E571S500 uRegular5 000-10 000 u/ml5,500.00 ₽
  • CNY: 429.49 ¥
E572L2500 uRegular5 000-10 000 u/ml22,000.00 ₽
  • CNY: 1,717.98 ¥

Description

Recognition site and hydrolysis position:

CAGNNNCTG CAGNNN↑CTG
GTCNNNGAC GTC↓NNNGAC

Source: Pseudomonas stutzeri 217
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
25 50 10 25 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme > 95% of Lambda DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: When using a buffer other than the optimal (suppied) SEBuffer, it may benecessary to add more enzyme to achieve complete digestion.
References:

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