RsaN I

1,500.00 ₽ – 6,000.00 ₽

  • CNY: 117.97 ¥ - 471.90 ¥

Restriction endonuclease RsaN I

  • Recognition site: G↑TAC / CAT↓G
  • Источник: Rhodopseudomonas sphaeroides N
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 80
SKU: SE-E555 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E555S200 uRegular5 000 u/ml1,500.00 ₽
  • CNY: 117.97 ¥
E556L1000 uRegular5 000 u/ml6,000.00 ₽
  • CNY: 471.90 ¥

Description

Recognition site and hydrolysis position:

GTAC G↑TAC
CATG CAT↓G

Source: Rhodopseudomonas sphaeroides N
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 75 50 50 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:

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