Set I

6,000.00 ₽ – 24,000.00 ₽

  • CNY: 468.54 ¥ - 1,874.16 ¥

Restriction endonuclease Set I

  • Recognition site: ASST↑ / ↓TSSA
  • Источник: Из штамма E.coli несущего клонированный ген SetI из Streptomyces werraensis 37
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 80
SKU: SE-E537 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E537S200 uRegular5 000 u/ml6,000.00 ₽
  • CNY: 468.54 ¥
E538L1000 uRegular5 000 u/ml24,000.00 ₽
  • CNY: 1,874.16 ¥
E538XL1000 uConcentrated20 000 u/ml24,000.00 ₽
  • CNY: 1,874.16 ¥

Description

Recognition site and hydrolysis position:

ASST ASST↑
TSSA ↓TSSA

Source: An E.coli strain that carries the cloned Set I gene from Streptomyces werraensis 37
Unit definition:
One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the below indicated structure in 1 hour at 55°C in a total reaction volume of 20 μl.
Assayed on:
Double-stranded oligonucleotide
5`-CGAGTTTATAGCTGGGCCCAAC-3`
3`-GCTCAAATATCGACCCGGGTTG-5`
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
25 25 75 75 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol; Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme, approximately 50% of the pBR322 DNA fragments can be ligated with T4 DNA Ligase and recut. Reagents Supplied with Enzyme 10 X SE-buffer Y
Non-specific hydrolisis:
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease SetI // In press

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