Description
Recognition site and hydrolysis position:
| CCCGGG | CCC↑GGG |
| GGGCCC | GGG↓CCC |
Source: An E.coli strain that carries the cloned Sma I gene from Serratia marcescens
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 25°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (HindIII-digest)
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):
| B | G | O | W | Y | ROSE |
| 0 | 0 | 0 | 0 | 100 | 50 |
Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated (by using of high concentration T4 DNA Ligase and 10% PEG) and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 25°C.Reagents Supplied with Enzyme
10 X SE-buffer Y.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Endow, S.A., Roberts, R.I., J. Mol. Biol. 112: 521-529 (1977).
