Sma I

4,400.00 ₽ – 17,600.00 ₽

  • CNY: 336.69 ¥ - 1,346.75 ¥

Restriction endonuclease Sma I

  • Recognition site: CCC↑GGG / GGG↓CCC
  • Источник: Из штамма E.coli несущего клонированный ген Sma I из Serratia marcescens
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 25
  • Температура инактивации, 20 мин при: 65
SKU: SE-E177 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E177S2000 uRegular20 000 u/ml4,400.00 ₽
  • CNY: 336.69 ¥
E178L10000 uRegular20 000 u/ml17,600.00 ₽
  • CNY: 1,346.75 ¥
E177TS2000 uTurbo20 000 u/ml4,400.00 ₽
  • CNY: 336.69 ¥
E178TL10000 uTurbo20 000 u/ml17,600.00 ₽
  • CNY: 1,346.75 ¥

Description

Recognition site and hydrolysis position:

CCCGGG CCC↑GGG
GGGCCC GGG↓CCC

Source: An E.coli strain that carries the cloned Sma I gene from Serratia marcescens
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 25°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (HindIII-digest)
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
0 0 0 0 100 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated (by using of high concentration T4 DNA Ligase and 10% PEG) and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 25°C. Reagents Supplied with Enzyme 10 X SE-buffer Y.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Endow, S.A., Roberts, R.I., J. Mol. Biol. 112: 521-529 (1977).

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