Sph I

4,400.00 17,600.00 

  • CNY: 357.81 ¥ - 1,431.23 ¥

Restriction endonuclease Sph I

  • Recognition site: GCATG↑C / C↓GTACG
  • Источник: Из штамма E.coli несущего клонированный ген Sph I из Streptomyces phaeochromogenes
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E129 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E129S500 uRegular5 000 u/ml4,400.00 
  • CNY: 357.81 ¥
E130L2500 uRegular5 000 u/ml17,600.00 
  • CNY: 1,431.23 ¥
E129TS500 uTurbo5 000 u/ml4,400.00 
  • CNY: 357.81 ¥
E130TL2500 uTurbo5 000 u/ml17,600.00 
  • CNY: 1,431.23 ¥

Description

Recognition site and hydrolysis position:

GCATGC GCATG↑C
CGTACG C↓GTACG

Source: An E.coli strain that carries the cloned Sph I gene from Streptomyces phaeochromogenes
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
25 100 75 75 50 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.Reagents Supplied with Enzyme

10 X SE-buffer G, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Fuchs, L.Y., Covarrubias, L., Escalante, L., Sanchez, S., Bolivar, F. Gene 10:39-46 (1980).