Sse9 I

2,200.00 8,800.00 

  • CNY: 178.90 ¥ - 715.62 ¥

Restriction endonuclease Sse9 I

  • Recognition site: ↑AATT / TTAA↓
  • Источник: Из штамма E.coli несущего клонированный ген Sse9 I из Sporosarcina species 9
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 65
SKU: SE-E217 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E217S500 uRegular5 000 u/ml2,200.00 
  • CNY: 178.90 ¥
E218L2500 uRegular5 000 u/ml8,800.00 
  • CNY: 715.62 ¥
E217TS500 uTurbo5 000 u/ml2,200.00 
  • CNY: 178.90 ¥
E218TL2500 uTurbo5 000 u/ml8,800.00 
  • CNY: 715.62 ¥

Description

Recognition site and hydrolysis position:

AATT ↑AATT
TTAA TTAA↓

Source: An E.coli strain that carries the cloned Sse9 I gene from Sporosarcina species
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
pBR322 DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 75 50 50 75 75

Storage conditions: 10 mM Tis-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer B, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: Blocked by methylation: 5`-A(m6A)TT-3`/ 3`-TT(m6A)A-5`.
Notes: At 37°C activity is 75% from maximum.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References:
Gonchar, D.A., Dedkov, V.S., Verkhozina, V.A., Kusner, Yu.S., Shevchenko, A.V., Degtyarev, S.Kh. Prikl. Biokhim. Mikrobiol. 34: 139-141 (1998).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006