TseF I

4,500.00 ₽ – 18,000.00 ₽

  • CNY: 351.67 ¥ - 1,406.70 ¥

Restriction endonuclease TseF I

  • Recognition site: ↑GTSAC / CASTG↓
  • Источник: Из штамма E.coli несущего клонированный ген TseFI из Thermus species F35
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 65
  • Температура инактивации, 20 мин при: N
SKU: SE-E589 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E589S200 uRegular5 000 u/ml4,500.00 ₽
  • CNY: 351.67 ¥
E590L1000 uRegular5 000 u/ml18,000.00 ₽
  • CNY: 1,406.70 ¥

Description

Recognition site and hydrolysis position:

GTSAC ↑GTSAC
CASTG CASTG↓

Source: An E.coli strain, that carries the cloned gene TseFI from Thermus species F35
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

B G O W Y ROSE
100 50 0 25 50 50

Storage conditions: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity:
Notes:
References:

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