Tth111 I

Tth111 I

Price range: 2 900 ₽ through 11 600 ₽

Restriction endonuclease Tth111 I

  • Recognition site:
    5'… GACN▼NNGTC …3'
    3'… CTGNN▲NCAG …5'
  • Source: An E.coli strain that carries the cloned gene Tth111I from Thermus Thermophilus 111
  • Optimal buffer: SE-buffer Y
  • Optimal temperature: 65 °C
  • Inactivated during 20 minutes at: 80 °C
SKU: SE-E097 Category:

SKUРackagePackageVariantConcentrationPrice 
E097S400 uRegular5 000 u/ml2 900 ₽
E098L2000 uRegular5 000 u/ml11 600 ₽

Description

Recognition site and hydrolysis position:

GACNNNGTC GACN↑NNGTC
CTGNNNCAG CTGNN↓NCAG

Source: An E.coli strain, that carries the cloned gene Tth111I from Thermus Thermophilus 111
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA (HindIII-digest)
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
75 50 10 10 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 500 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme 10% of the DNA fragments can be ligated.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentrationmay result in star activity.
References:
Shinomiya, T., Sato, S. Nucleic Acids Res. 8: 43-56 (1980).

Additional Information

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