SibEnzyme restriction enzymes database

Список ферментов

AccB1 I

Enzyme name AccB1 I
Prototype HgiCI
SKU SE-E163
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G▼GYRCC …3'
3'… CCRYG▲G …5'
Source Acinetobacter calcoaceticus B1
Optimal buffer SE-buffer K + BSA
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
501010755030
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer K, BSA
Notes High enzyme concentration may result in star activity To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Degtyarev, S.Kh., Dedkov, V.S., Belichenko, O.A., Verchosina, V.A., Kusner, Y.S., Unpublished observations.

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AccB1 I

Enzyme name AccB1 I
Prototype HgiCI
SKU SE-E163
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G▼GYRCC …3'
3'… CCRYG▲G …5'
Source Acinetobacter calcoaceticus B1
Optimal buffer SE-buffer K + BSA
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
501010755030
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer K, BSA
Notes High enzyme concentration may result in star activity To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Degtyarev, S.Kh., Dedkov, V.S., Belichenko, O.A., Verchosina, V.A., Kusner, Y.S., Unpublished observations.