SibEnzyme restriction enzymes database

Список ферментов

Acl I

Enzyme name Acl I
Prototype AclI
SKU SE-E011
Turbo version Not available
High-concentration version Not available
Recognition site
5'… AA▼CGTT …3'
3'… TTGC▲AA …5'
Source Acinetobacter calcoaceticus
Optimal buffer SE-buffer Y + BSA
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
000010080
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 0.05% Triton X-100; 50% glycerol; Store at -20°C.
Ligation After 2-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Degtyarev, S.Kh., Abdurashitov, M.A., Kolyhalov, A.A., Rechkunova, N.I., Nucleic Acids Res. 20: 3787 (1992).

Поиск по названию

Acl I

Enzyme name Acl I
Prototype AclI
SKU SE-E011
Turbo version Not available
High-concentration version Not available
Recognition site
5'… AA▼CGTT …3'
3'… TTGC▲AA …5'
Source Acinetobacter calcoaceticus
Optimal buffer SE-buffer Y + BSA
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
000010080
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 0.05% Triton X-100; 50% glycerol; Store at -20°C.
Ligation After 2-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Degtyarev, S.Kh., Abdurashitov, M.A., Kolyhalov, A.A., Rechkunova, N.I., Nucleic Acids Res. 20: 3787 (1992).