SibEnzyme restriction enzymes database

Список ферментов

Ahl I

Enzyme name Ahl I
Prototype SpeI
SKU SE-E173
Turbo version Available
High-concentration version Not available
Recognition site
5'… A▼CTAGT …3'
3'… TGATC▲A …5'
Source Alteromonas haloplanktis SP
Optimal buffer SE-buffer B + BSA
Optimal temperature 37 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
10075252575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of T7 DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Myakisheva, T.V., Belichenko, O.A., Popichenko, D.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (2000).

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Ahl I

Enzyme name Ahl I
Prototype SpeI
SKU SE-E173
Turbo version Available
High-concentration version Not available
Recognition site
5'… A▼CTAGT …3'
3'… TGATC▲A …5'
Source Alteromonas haloplanktis SP
Optimal buffer SE-buffer B + BSA
Optimal temperature 37 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
10075252575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of T7 DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Myakisheva, T.V., Belichenko, O.A., Popichenko, D.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (2000).