SibEnzyme restriction enzymes database

Список ферментов

AsiG I

Enzyme name AsiG I
Prototype AgeI
SKU SE-E235
Turbo version Available
High-concentration version Not available
Recognition site
5'… A▼CCGGT …3'
3'… TGGCC▲A …5'
Source Arthrobacter species G
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1025100751075
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O.
Notes Turbo AsiG I can be used for short time (10 min) DNA digestion as well as for standard reaction. The reaction can be performed using optimal or universal (ROSE) Buffer.
References
  1. Chmuzh, E.V., Mikhnenkova, N.A., Nayakshina, T.N., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations.(2005)

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AsiG I

Enzyme name AsiG I
Prototype AgeI
SKU SE-E235
Turbo version Available
High-concentration version Not available
Recognition site
5'… A▼CCGGT …3'
3'… TGGCC▲A …5'
Source Arthrobacter species G
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1025100751075
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O.
Notes Turbo AsiG I can be used for short time (10 min) DNA digestion as well as for standard reaction. The reaction can be performed using optimal or universal (ROSE) Buffer.
References
  1. Chmuzh, E.V., Mikhnenkova, N.A., Nayakshina, T.N., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations.(2005)