SibEnzyme restriction enzymes database

Список ферментов

BstF5 I

Enzyme name BstF5 I
Prototype (FokI)
SKU SE-E031
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
Source Bacillus stearothermophilus F5
Optimal buffer SE-buffer Y
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75502550100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.
References
  1. Abdurashitov, M.A., Kileva, E.V., Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Gene 172: 49-51 (1996).

Поиск по названию

BstF5 I

Enzyme name BstF5 I
Prototype (FokI)
SKU SE-E031
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
Source Bacillus stearothermophilus F5
Optimal buffer SE-buffer Y
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75502550100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.
References
  1. Abdurashitov, M.A., Kileva, E.V., Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Gene 172: 49-51 (1996).