SibEnzyme restriction enzymes database

Список ферментов

BstSF I

Enzyme name BstSF I
Prototype SfeI
SKU SE-E197
Turbo version Not available
High-concentration version Not available
Recognition site
5'… C▼TRYAG …3'
3'… GAYRT▲C …5'
Source Bacillus stearothermophilus SF
Optimal buffer SE-buffer O + BSA
Optimal temperature 60 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
75251005050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).

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BstSF I

Enzyme name BstSF I
Prototype SfeI
SKU SE-E197
Turbo version Not available
High-concentration version Not available
Recognition site
5'… C▼TRYAG …3'
3'… GAYRT▲C …5'
Source Bacillus stearothermophilus SF
Optimal buffer SE-buffer O + BSA
Optimal temperature 60 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
75251005050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).