SibEnzyme restriction enzymes database

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Ege I

Enzyme name Ege I
Prototype NarI
SKU SE-E243
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGC▼GCC …3'
3'… CCG▲CGG …5'
Source Enterobacter gergoviae
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10075105075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycero; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Dedkov, V.S., Myakisheva, T.V., Kileva, E.V., Degtyarev, S.K. Unpublished observations (1996)

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Ege I

Enzyme name Ege I
Prototype NarI
SKU SE-E243
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGC▼GCC …3'
3'… CCG▲CGG …5'
Source Enterobacter gergoviae
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10075105075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycero; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Dedkov, V.S., Myakisheva, T.V., Kileva, E.V., Degtyarev, S.K. Unpublished observations (1996)