SibEnzyme restriction enzymes database

Список ферментов

Fok I

Enzyme name Fok I
Prototype FokI
SKU SE-E247
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGATG(N)9▼ …3'
3'… CCTAC(N)13▲ …5'
Source An E.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50502525100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 1 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes Overdigestions of > 5 units of Fok I per 1μg of DNA and incubations > 2 hours are not recommended.
References
  1. Sugisaki, H., Kanazawa, S., Gene 16: 73-78 (1981).

Поиск по названию

Fok I

Enzyme name Fok I
Prototype FokI
SKU SE-E247
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGATG(N)9▼ …3'
3'… CCTAC(N)13▲ …5'
Source An E.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50502525100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 1 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes Overdigestions of > 5 units of Fok I per 1μg of DNA and incubations > 2 hours are not recommended.
References
  1. Sugisaki, H., Kanazawa, S., Gene 16: 73-78 (1981).